An extra-plastidial -glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation
Identifieur interne : 002359 ( Main/Exploration ); précédent : 002358; suivant : 002360An extra-plastidial -glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation
Auteurs : Mikkel A. Glaring [Danemark] ; Agnieszka Zygadlo [Danemark] ; David Thorneycroft [Royaume-Uni] ; Alexander Schulz [Danemark] ; Steven M. Smith [Royaume-Uni, Australie] ; Andreas Blennow [Danemark] ; Lone Baunsgaard [Danemark]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2007-11-17.
Abstract
Starch phosphorylation catalysed by the -glucan, water dikinases (GWD) has profound effects on starch degradation in plants. The Arabidopsis thaliana genome encodes three isoforms of GWD, two of which are localized in the chloroplast and are involved in the degradation of transient starch. The third isoform, termed AtGWD2 (At4g24450), was heterologously expressed and purified and shown to have a substrate preference similar to potato GWD. Analyses of AtGWD2 null mutants did not reveal any differences in growth or starch and sugar levels, when compared to the wild type. Subcellular localization studies in Arabidopsis leaves and in vitro chloroplast import assays indicated that AtGWD2 was not targeted to the chloroplasts. The AtGWD2 promoter showed a highly restricted pattern of activity, both spatially and temporally. High activity was observed in the companion cells of the phloem, with expression appearing just before the onset of senescence. Taken together, these data indicate that, although AtGWD2 is capable of phosphorylating -glucans in vitro, it is not directly involved in transient starch degradation.
Url:
DOI: 10.1093/jxb/erm249
Affiliations:
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The Arabidopsis thaliana genome encodes three isoforms of GWD, two of which are localized in the chloroplast and are involved in the degradation of transient starch. The third isoform, termed AtGWD2 (At4g24450), was heterologously expressed and purified and shown to have a substrate preference similar to potato GWD. Analyses of AtGWD2 null mutants did not reveal any differences in growth or starch and sugar levels, when compared to the wild type. Subcellular localization studies in Arabidopsis leaves and in vitro chloroplast import assays indicated that AtGWD2 was not targeted to the chloroplasts. The AtGWD2 promoter showed a highly restricted pattern of activity, both spatially and temporally. High activity was observed in the companion cells of the phloem, with expression appearing just before the onset of senescence. Taken together, these data indicate that, although AtGWD2 is capable of phosphorylating -glucans in vitro, it is not directly involved in transient starch degradation.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Danemark</li><li>Royaume-Uni</li></country><region><li>Hovedstaden</li><li>Écosse</li></region><settlement><li>Copenhague</li><li>Édimbourg</li></settlement><orgName><li>Université d'Édimbourg</li></orgName></list><tree><country name="Danemark"><region name="Hovedstaden"><name sortKey="Glaring, Mikkel A" sort="Glaring, Mikkel A" uniqKey="Glaring M" first="Mikkel A." last="Glaring">Mikkel A. 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Glaring</name><name sortKey="Schulz, Alexander" sort="Schulz, Alexander" uniqKey="Schulz A" first="Alexander" last="Schulz">Alexander Schulz</name><name sortKey="Zygadlo, Agnieszka" sort="Zygadlo, Agnieszka" uniqKey="Zygadlo A" first="Agnieszka" last="Zygadlo">Agnieszka Zygadlo</name></country><country name="Royaume-Uni"><region name="Écosse"><name sortKey="Thorneycroft, David" sort="Thorneycroft, David" uniqKey="Thorneycroft D" first="David" last="Thorneycroft">David Thorneycroft</name></region><name sortKey="Smith, Steven M" sort="Smith, Steven M" uniqKey="Smith S" first="Steven M." last="Smith">Steven M. Smith</name></country><country name="Australie"><noRegion><name sortKey="Smith, Steven M" sort="Smith, Steven M" uniqKey="Smith S" first="Steven M." last="Smith">Steven M. Smith</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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